Abstract
We report a novel method for the selective proteolysis by limiting protease access to the substrate, which we have named nano-surface and molecular-orientation limited (nSMOL) proteolysis. We focus on the identification of the Fab and quantitation of antibodies. This method successfully performed limited proteolysis on the Fab.
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / chemistry*
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Antibodies, Monoclonal / metabolism*
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Complementarity Determining Regions / chemistry
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Complementarity Determining Regions / metabolism*
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Models, Molecular
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Molecular Sequence Data
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Nanotechnology / methods*
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Protein Conformation
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Proteolysis*
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Surface Properties
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Trypsin / metabolism*
Substances
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Antibodies, Monoclonal
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Complementarity Determining Regions
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Trypsin