Whole exome sequencing of insulinoma reveals recurrent T372R mutations in YY1

Nat Commun. 2013;4:2810. doi: 10.1038/ncomms3810.

Abstract

Functional pancreatic neuroendocrine tumours (PNETs) are mainly represented by insulinoma, which secrete insulin independent of glucose and cause hypoglycaemia. The major genetic alterations in sporadic insulinomas are still unknown. Here we identify recurrent somatic T372R mutations in YY1 by whole exome sequencing of 10 sporadic insulinomas. Further screening in 103 additional insulinomas reveals this hotspot mutation in 30% (34/113) of all tumours. T372R mutation alters the expression of YY1 target genes in insulinomas. Clinically, the T372R mutation is associated with the later onset of tumours. Genotyping of YY1, a target of mTOR inhibitors, may contribute to medical treatment of insulinomas. Our findings highlight the importance of YY1 in pancreatic β-cells and may provide therapeutic targets for PNETs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Case-Control Studies
  • Cattle
  • Cell Line
  • Exome
  • Gene Expression Regulation, Neoplastic
  • Gene Targeting
  • Humans
  • Insulinoma / genetics*
  • Mice
  • Molecular Sequence Data
  • Mutation, Missense
  • Pancreatic Neoplasms / genetics*
  • Rats
  • YY1 Transcription Factor / genetics*
  • YY1 Transcription Factor / metabolism

Substances

  • YY1 Transcription Factor
  • YY1 protein, human