The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining

Am J Pathol. 1986 Dec;125(3):431-5.


A new simplified tissue processing method for immunostaining was devised. Tissues were fixed in acetone at -20 C overnight, then cleared in methyl benzoate and xylene, consecutively, and embedded in ordinary paraffin at 58-60 C. Thin paraffin sections were deparaffinized with xylene, immersed in acetone and then phosphate buffered saline, and immunostained with various monoclonal and conventional antibodies, which have only been used on fresh-frozen or PLP-fixed frozen sections. As with PLP-fixed frozen sections, the following antigens were clearly demonstrated in the tissue sections processed with the present method: T (Leu-1, Leu-2a, Leu-3a, Leu-4, OKT3, OKT4, OKT8), B (B1, B2, Leu-14, IgM, IgD) lymphocyte surface markers and other antigens (Leu-7, OKT6, OKT9, OKM1, OKI1, J5, Ki-1, Ki-67, TdT, oncogene Ha-ras P21). Sections prepared by the present method demonstrated much better histologic and cytologic preservation than possible in frozen sections.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetone*
  • Antigens / analysis*
  • Benzoates*
  • Fixatives
  • Frozen Sections
  • Histological Techniques*
  • Humans
  • Paraffin
  • Staining and Labeling
  • Xylenes*


  • Antigens
  • Benzoates
  • Fixatives
  • Xylenes
  • Acetone
  • methyl benzoate
  • Paraffin