Human cell-based artificial antigen-presenting cells for cancer immunotherapy

Immunol Rev. 2014 Jan;257(1):191-209. doi: 10.1111/imr.12129.


Adoptive T-cell therapy, where anti-tumor T cells are first prepared in vitro, is attractive since it facilitates the delivery of essential signals to selected subsets of anti-tumor T cells without unfavorable immunoregulatory issues that exist in tumor-bearing hosts. Recent clinical trials have demonstrated that anti-tumor adoptive T-cell therapy, i.e. infusion of tumor-specific T cells, can induce clinically relevant and sustained responses in patients with advanced cancer. The goal of adoptive cell therapy is to establish anti-tumor immunologic memory, which can result in life-long rejection of tumor cells in patients. To achieve this goal, during the process of in vitro expansion, T-cell grafts used in adoptive T-cell therapy must be appropriately educated and equipped with the capacity to accomplish multiple, essential tasks. Adoptively transferred T cells must be endowed, prior to infusion, with the ability to efficiently engraft, expand, persist, and traffic to tumor in vivo. As a strategy to consistently generate T-cell grafts with these capabilities, artificial antigen-presenting cells have been developed to deliver the proper signals necessary to T cells to enable optimal adoptive cell therapy.

Keywords: K562; adoptive therapy; artificial antigen-presenting cell; cytokine; cytotoxic T cell; memory.

Publication types

  • Review

MeSH terms

  • Animals
  • Antigen-Presenting Cells / cytology
  • Antigen-Presenting Cells / immunology*
  • Antigen-Presenting Cells / metabolism
  • Cell Culture Techniques
  • Clinical Trials as Topic
  • Combined Modality Therapy
  • Cytokines / metabolism
  • Cytokines / pharmacology
  • Genetic Engineering*
  • Humans
  • Immunologic Memory
  • Immunotherapy, Adoptive* / methods
  • K562 Cells / immunology
  • K562 Cells / metabolism
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism
  • Neoplasms / immunology*
  • Neoplasms / therapy*
  • Phenotype
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / metabolism
  • Recombinant Fusion Proteins
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism


  • Cytokines
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins