Differential expression and elution behavior of basic 7S globulin among cultivars under hot water treatment of soybean seeds

J Biosci Bioeng. 2014 Jun;117(6):742-8. doi: 10.1016/j.jbiosc.2013.11.004. Epub 2013 Dec 9.

Abstract

Basic 7S globulin (Bg7S), which accumulates in mature soybean (Glycine max) seeds, is an extracellular matrix protein. A large amount of Bg7S is synthesized de novo and is eluted from soybean seeds when immersed in 50-60°C water (hot water treatment, HWT). However, the Bg7S elution mechanism remains unclear. Under HWT, the seeds probably undergo heat stress and flooding stress. To obtain fundamental knowledge related to how Bg7S is eluted from hot-water-treated seeds, this study compared Bg7S elution among soybean cultivars having different flooding tolerance during pre-germination. The amounts of Bg7S eluted from seeds varied significantly among cultivars. Elution was suppressed by seed coats regarded as preventing the leakage of seed contents by rapid water imbibition. Furthermore, Bg7S expression levels differed among cultivars, although the difference did not result from any variation in Bg7S promoter sequences. However, the expression levels of Bg7S under HWT were not associated with the flooding tolerance level. Immunoelectron microscopy revealed that the Bg7S accumulated in the intercellular space of hot-water-treated seeds. Plasma membrane shrinkage was observed. The main proteins eluted from seeds under HWT were located in the extracellular space. This study clarified the mechanism of Bg7S elution from seeds under HWT.

Keywords: Basic 7S globulin; Seed; Seed coat; Soybean; Stress response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Plant / biosynthesis*
  • Antigens, Plant / genetics
  • Base Sequence
  • Globulins / biosynthesis*
  • Globulins / genetics
  • Globulins / metabolism
  • Heat-Shock Response
  • Promoter Regions, Genetic
  • Protein Transport
  • Seed Storage Proteins / biosynthesis*
  • Seed Storage Proteins / genetics
  • Seed Storage Proteins / metabolism
  • Seeds / metabolism*
  • Seeds / ultrastructure
  • Sequence Analysis, Protein
  • Soybean Proteins / biosynthesis*
  • Soybean Proteins / genetics
  • Soybean Proteins / metabolism
  • Soybeans / metabolism*
  • Soybeans / ultrastructure

Substances

  • Antigens, Plant
  • Globulins
  • Seed Storage Proteins
  • Soybean Proteins
  • beta-conglycinin protein, Glycine max