Systemic upregulation of PDGF-B in patients with neovascular AMD

Invest Ophthalmol Vis Sci. 2014 Jan 20;55(1):337-44. doi: 10.1167/iovs.13-12978.


Purpose: To determine the plasma levels of platelet-derived growth factor-B (PDGF-B), VEGF, and TNF-α in patients with neovascular AMD and in patients with diabetic macular edema (DME).

Methods: Thirty patients with neovascular AMD, 30 patients with DME, and 12 healthy controls were included in this prospective study. The concentrations of PDGF-B, VEGF, and TNF-α were measured by ELISA.

Results: The PDGF-B concentration in the plasma of controls was (median [25th-75th percentile]) 263.5 (162.0-513.3) pg/mL and in patients with DME 219.0 (122.8-604.8) pg/mL. In patients with neovascular AMD, PDGF-B levels were significantly higher with a median plasma concentration of 783.5 (289.3-1183.5) pg/mL (P = 0.003). The VEGF concentrations in patients with DME 33.0 (21.8-73.0) pg/mL and in patients with neovascular AMD 55.0 (37.0-116.3) pg/mL showed no significant differences (P = 0.159). A positive correlation of PDGF-B and VEGF plasma levels was found in patients with neovascular AMD and in patients with DME (r = 0.683, P < 0.001, and r = 0.612, P < 0.001, respectively). No significant differences of systemic TNF-α levels could be found between the three study groups.

Conclusions: Patients with neovascular AMD have significantly higher plasma PDGF-B levels compared with patients with DME and healthy controls. Our study data indicate that PDGF-B may be involved in the pathogenesis of neovascular AMD. ( number, EudraCT 2010-024654-11)

Keywords: HIF-1; PDGF-B; TNF-α; diabetic retinopathy; neovascular age-related macular degeneration; plasma levels.

Publication types

  • Comparative Study

MeSH terms

  • Biomarkers / blood
  • Diabetic Retinopathy / blood
  • Diabetic Retinopathy / complications
  • Diabetic Retinopathy / diagnosis
  • Enzyme-Linked Immunosorbent Assay
  • Follow-Up Studies
  • Humans
  • Macular Degeneration / blood*
  • Macular Degeneration / complications
  • Macular Degeneration / diagnosis
  • Macular Edema / blood
  • Macular Edema / complications
  • Macular Edema / diagnosis
  • Prospective Studies
  • Proto-Oncogene Proteins c-sis / blood*
  • Retinal Neovascularization / blood*
  • Retinal Neovascularization / complications
  • Retinal Neovascularization / diagnosis
  • Tumor Necrosis Factor-alpha / blood
  • Up-Regulation*
  • Vascular Endothelial Growth Factor A / blood


  • Biomarkers
  • Proto-Oncogene Proteins c-sis
  • Tumor Necrosis Factor-alpha
  • Vascular Endothelial Growth Factor A