Sensing acetylcholine and anticholinesterase compounds

Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1302-5. doi: 10.1002/anie.201307754. Epub 2013 Dec 13.

Abstract

Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, and chemical warfare agents. A semisynthetic fluorescence-based probe for the direct, real-time detection of acetylcholine and anticholinesterase compounds is introduced. The probe possesses good sensitivity, tunable detection range, and can be selectively targeted to cell surfaces, thereby making it an attractive tool for applications in analytical chemistry and quantitative biology.

Keywords: FRET; acetylcholine; acetylcholinesterase; biosensors; imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / analysis*
  • Acetylcholinesterase / chemistry*
  • Acetylcholinesterase / genetics
  • Acetylcholinesterase / metabolism
  • Biosensing Techniques*
  • Chemical Warfare Agents / analysis
  • Cholinesterase Inhibitors / analysis
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / chemistry
  • HEK293 Cells
  • Humans
  • Microscopy, Confocal
  • Pesticides / analysis

Substances

  • Chemical Warfare Agents
  • Cholinesterase Inhibitors
  • Fluorescent Dyes
  • Pesticides
  • Acetylcholinesterase
  • Acetylcholine