Not all that glitters is gold: off-target recombination in the somatostatin-IRES-Cre mouse line labels a subset of fast-spiking interneurons

doi:10.3389/fncir.2013.00195

. 2013 Dec 10:7:195.

doi: 10.3389/fncir.2013.00195. eCollection 2013.

Not all that glitters is gold: off-target recombination in the somatostatin-IRES-Cre mouse line labels a subset of fast-spiking interneurons

Hang Hu¹, John Z Cavendish, Ariel Agmon

Affiliations

PMID: 24339803
PMCID: PMC3857604
DOI: 10.3389/fncir.2013.00195

Not all that glitters is gold: off-target recombination in the somatostatin-IRES-Cre mouse line labels a subset of fast-spiking interneurons

Hang Hu et al. Front Neural Circuits. 2013.

. 2013 Dec 10:7:195.

doi: 10.3389/fncir.2013.00195. eCollection 2013.

Authors

Hang Hu¹, John Z Cavendish, Ariel Agmon

Affiliation

¹ Department of Neurobiology and Anatomy and the Sensory Neuroscience Research Center, West Virginia University Morgantown, WV, USA.

PMID: 24339803
PMCID: PMC3857604
DOI: 10.3389/fncir.2013.00195

No abstract available

Keywords: Cre mice; Cre reporters; fast spiking; inhibitory interneurons; parvalbumin; somatostatin.

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Figures

**Figure 1**
**RFP-expressing fast-spiking interneurons in SOM-RFP mice. (A)** Voltage responses (upper panels) of an RFP-expressing layer 5 barrel cortex neuron to the intracellular current steps shown in the lower panel. Note the pronounced after-hyperpolarization (arrowhead) and the non-adapting spike train, typical of FS interneurons. **(B)** The same neuron was found *post-hoc* to contain biocytin and RFP and to be immunopositive for PV. **(C)** A projection of a confocal stack from cortical area A1, showing five RFP-expressing cells immunopositive for PV (circled). The cells labeled #1 and #2 are shown in **(D)**. **(D)** Single optical sections from subregions of the same field of view as in **(C)**, in the vicinity of cell #1 (left panels, arrows) and cell #2 (right panels, arrows), separated into red and green channels.

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References

1. Adesnik H., Bruns W., Taniguchi H., Huang Z. J., Scanziani M. (2012). A neural circuit for spatial summation in visual cortex. Nature 490, 226–231 10.1038/nature11526 - DOI - PMC - PubMed
1. Batista-Brito R., Fishell G. (2009). Chapter 3 the developmental integration of cortical interneurons into a functional network. Curr. Top. Dev. Biol. 87, 81–118 10.1016/S0070-2153(09)01203-4 - DOI - PMC - PubMed
1. Beierlein M., Gibson J. R., Connors B. W. (2003). Two dynamically distinct inhibitory networks in layer 4 of the neocortex. J. Neurophysiol. 90, 2987–3000 10.1152/jn.00283.2003 - DOI - PubMed
1. Chattopadhyaya B., Di Cristo G., Higashiyama H., Knott G. W., Kuhlman S. J., Welker E., et al. (2004). Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. J. Neurosci. 24, 9598–9611 10.1523/JNEUROSCI.1851-04.2004 - DOI - PMC - PubMed
1. Chiu C. Q., Lur G., Morse T. M., Carnevale N. T., Ellis-Davies G. C., Higley M. J. (2013). Compartmentalization of GABAergic inhibition by dendritic spines. Science 340, 759–762 10.1126/science.1234274 - DOI - PMC - PubMed

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[1] Adesnik H., Bruns W., Taniguchi H., Huang Z. J., Scanziani M. (2012). A neural circuit for spatial summation in visual cortex. Nature 490, 226–231 10.1038/nature11526 - DOI - PMC - PubMed

[2] Adesnik H., Bruns W., Taniguchi H., Huang Z. J., Scanziani M. (2012). A neural circuit for spatial summation in visual cortex. Nature 490, 226–231 10.1038/nature11526 - DOI - PMC - PubMed

[3] Batista-Brito R., Fishell G. (2009). Chapter 3 the developmental integration of cortical interneurons into a functional network. Curr. Top. Dev. Biol. 87, 81–118 10.1016/S0070-2153(09)01203-4 - DOI - PMC - PubMed

[4] Batista-Brito R., Fishell G. (2009). Chapter 3 the developmental integration of cortical interneurons into a functional network. Curr. Top. Dev. Biol. 87, 81–118 10.1016/S0070-2153(09)01203-4 - DOI - PMC - PubMed

[5] Beierlein M., Gibson J. R., Connors B. W. (2003). Two dynamically distinct inhibitory networks in layer 4 of the neocortex. J. Neurophysiol. 90, 2987–3000 10.1152/jn.00283.2003 - DOI - PubMed

[6] Beierlein M., Gibson J. R., Connors B. W. (2003). Two dynamically distinct inhibitory networks in layer 4 of the neocortex. J. Neurophysiol. 90, 2987–3000 10.1152/jn.00283.2003 - DOI - PubMed

[7] Chattopadhyaya B., Di Cristo G., Higashiyama H., Knott G. W., Kuhlman S. J., Welker E., et al. (2004). Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. J. Neurosci. 24, 9598–9611 10.1523/JNEUROSCI.1851-04.2004 - DOI - PMC - PubMed

[8] Chattopadhyaya B., Di Cristo G., Higashiyama H., Knott G. W., Kuhlman S. J., Welker E., et al. (2004). Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. J. Neurosci. 24, 9598–9611 10.1523/JNEUROSCI.1851-04.2004 - DOI - PMC - PubMed

[9] Chiu C. Q., Lur G., Morse T. M., Carnevale N. T., Ellis-Davies G. C., Higley M. J. (2013). Compartmentalization of GABAergic inhibition by dendritic spines. Science 340, 759–762 10.1126/science.1234274 - DOI - PMC - PubMed

[10] Chiu C. Q., Lur G., Morse T. M., Carnevale N. T., Ellis-Davies G. C., Higley M. J. (2013). Compartmentalization of GABAergic inhibition by dendritic spines. Science 340, 759–762 10.1126/science.1234274 - DOI - PMC - PubMed

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Not all that glitters is gold: off-target recombination in the somatostatin-IRES-Cre mouse line labels a subset of fast-spiking interneurons

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Not all that glitters is gold: off-target recombination in the somatostatin-IRES-Cre mouse line labels a subset of fast-spiking interneurons

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