Differential gene expression of cardiac ion channels in human dilated cardiomyopathy

PLoS One. 2013 Dec 5;8(12):e79792. doi: 10.1371/journal.pone.0079792. eCollection 2013.

Abstract

Background: Dilated cardiomyopathy (DCM) is characterized by idiopathic dilation and systolic contractile dysfunction of the cardiac chambers. The present work aimed to study the alterations in gene expression of ion channels involved in cardiomyocyte function.

Methods and results: Microarray profiling using the Affymetrix Human Gene® 1.0 ST array was performed using 17 RNA samples, 12 from DCM patients undergoing cardiac transplantation and 5 control donors (CNT). The analysis focused on 7 cardiac ion channel genes, since this category has not been previously studied in human DCM. SCN2B was upregulated, while KCNJ5, KCNJ8, CLIC2, CLCN3, CACNB2, and CACNA1C were downregulated. The RT-qPCR (21 DCM and 8 CNT samples) validated the gene expression of SCN2B (p < 0.0001), KCNJ5 (p < 0.05), KCNJ8 (p < 0.05), CLIC2 (p < 0.05), and CACNB2 (p < 0.05). Furthermore, we performed an IPA analysis and we found a functional relationship between the different ion channels studied in this work.

Conclusion: This study shows a differential expression of ion channel genes involved in cardiac contraction in DCM that might partly underlie the changes in left ventricular function observed in these patients. These results could be the basis for new genetic therapeutic approaches.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cardiomyopathy, Dilated / genetics*
  • Cardiomyopathy, Dilated / metabolism*
  • Case-Control Studies
  • Female
  • Humans
  • Ion Channels / genetics*
  • Ion Channels / metabolism*
  • Male
  • Middle Aged
  • Myocardium / metabolism*
  • Transcriptome*

Substances

  • Ion Channels

Grant support

This work was supported by grants of the National Institute of Health “Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III” [RD12/0042/0003; FIS Project PI10/00275]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.