Glucose-induced changes in cytosolic ATP content in pancreatic islets

Biochim Biophys Acta. 1987 Feb 18;927(2):190-5. doi: 10.1016/0167-4889(87)90134-0.

Abstract

The cytosolic and mitochondrial contents in ATP, ADP and AMP were measured in islets incubated for 45 min at increasing concentrations of D-glucose and then exposed for 20 s to digitonin. The latter treatment failed to affect the total islet ATP/ADP ratio and adenylate charge. D-Glucose caused a much greater increase in cytosolic than mitochondrial ATP/ADP ratio. In the cytosol, a sigmoidal pattern characterized the changes in ATP/ADP ratio at increasing concentrations of D-glucose. These findings are compatible with the view that cytosolic ATP participates in the coupling of metabolic to ionic events in the process of nutrient-induced insulin release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / analysis
  • Adenosine Monophosphate / analysis
  • Adenosine Triphosphate / analysis*
  • Animals
  • Cytosol / analysis
  • Digitonin / pharmacology
  • Glucose / pharmacology*
  • Glutamate Dehydrogenase / metabolism
  • Insulin / metabolism
  • Insulin Secretion
  • Ion Channels / drug effects
  • Islets of Langerhans / analysis
  • Islets of Langerhans / drug effects*
  • Islets of Langerhans / metabolism
  • L-Lactate Dehydrogenase / metabolism
  • Mitochondria / analysis
  • Potassium / metabolism
  • Rats

Substances

  • Insulin
  • Ion Channels
  • Adenosine Monophosphate
  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • L-Lactate Dehydrogenase
  • Glutamate Dehydrogenase
  • Glucose
  • Digitonin
  • Potassium