Modified technique for cytochrome oxidase histochemistry: increased staining intensity and compatibility with 2-deoxyglucose autoradiography

J Neurosci Methods. 1987 Jan;19(1):1-10. doi: 10.1016/0165-0270(87)90016-1.


Staining the brain for cytochrome oxidase (CO) produces patterns which can resemble the patterns produced by 2-deoxyglucose (2-DG) autoradiography. In order to assess the differences between CO patterns of long-term metabolic activity and 2-DG patterns of short-term activity, comparisons should, ideally, be made on the same section. Consequently, we have made certain modifications in the standard CO histologic procedure which improve the stain and allow both 2-DG autoradiography and CO staining on the same section.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Brain / enzymology*
  • Brain / metabolism
  • Cerebellum / metabolism
  • Deoxy Sugars / metabolism*
  • Deoxyglucose / metabolism*
  • Electron Transport Complex IV / metabolism*
  • Histocytochemistry / methods*
  • Rats
  • Rats, Inbred Strains
  • Staining and Labeling / methods


  • Deoxy Sugars
  • Deoxyglucose
  • Electron Transport Complex IV