Induction of various HLA class II molecules in a human colonic adenocarcinoma cell line

Scand J Immunol. 1987 Feb;25(2):175-80. doi: 10.1111/j.1365-3083.1987.tb01061.x.

Abstract

The colonic carcinoma cell line HT-29 had no constitutive expression of HLA class II molecules. Gamma interferon (IFN-gamma) induced expression of HLA class II molecules in a dose-dependent manner with 100 U/ml as an optimal dose. The expression of HLA-DR, HLA-DP, and HLA-DQ molecules seemed to follow different kinetics. While DR and DP molecules were maximally induced after 2 days, DQ molecules appeared later with maximum percentage positive cells after 8 days. Treatment with a prostaglandin synthesis inhibitor (indomethacin) neither induced class II expression nor altered the dose-response curve for IFN-gamma; this indicated that possible endogenous production of prostaglandins in this cell line did not interfere with its class II expression. The lectins phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and wheat germ agglutinin (WGA) did not induce class II expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / immunology
  • Adenocarcinoma / pathology*
  • Cell Line
  • Colonic Neoplasms / immunology
  • Colonic Neoplasms / pathology*
  • Epitopes / physiology
  • HLA-D Antigens / immunology*
  • Humans
  • Interferon-gamma / pharmacology
  • Intestine, Small / immunology

Substances

  • Epitopes
  • HLA-D Antigens
  • Interferon-gamma