Bombesin stimulation of gastrin release from canine gastrin cells in primary culture

Am J Physiol. 1987 Mar;252(3 Pt 1):G413-20. doi: 10.1152/ajpgi.1987.252.3.G413.


A method is described for the isolation and short-term culture of canine antral gastrin (G) cells. Tissue was dispersed by enzymes and G cells enriched by elutriation and cultured for 40 h. These cultures contained 12% G cells and less than 2% somatostatin- or serotonin-containing cells. Bombesin (0.001-100 pM) potently stimulated gastrin release from cell cultures in a linear fashion over 2 h. The bombesin-specific monoclonal antibody 2A11 dose-dependently blocked bombesin stimulation. Somatostatin (0.001-1,000 nM) inhibited bombesin-stimulated gastrin release. Antibody to somatostatin (Mab S8) prevented the inhibition by exogenous somatostatin but did not alter bombesin-stimulated or basal gastrin release. The substance P (SP) analogue spantide (1 nM-1 microM) did not inhibit bombesin-stimulated gastrin release. Postreceptor activation of adenylate cyclase by forskolin and of protein kinase C by the phorbol ester, beta-TPA, caused gastrin release. The calcium ionophore A23187 also released gastrin in a dose-dependent fashion. This methodology allows enrichment and short-term culture of antral G cells; these cells have stimulatory bombesin and inhibitory somatostatin receptors, suggesting that these peptides have a direct action on antral G cells. Furthermore, G cells are activated by cAMP and calcium/phosphatidylinositol-dependent mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Animals
  • Bombesin / antagonists & inhibitors
  • Bombesin / pharmacology*
  • Calcimycin / pharmacology
  • Cells, Cultured
  • Colforsin / pharmacology
  • Dogs
  • Gastric Juice / metabolism*
  • Gastric Mucosa / metabolism
  • Gastrins / metabolism*
  • Protein Kinase C / metabolism
  • Receptors, Bombesin
  • Receptors, Neurotransmitter / physiology
  • Secretory Rate / drug effects
  • Somatostatin / pharmacology
  • Substance P / analogs & derivatives
  • Substance P / pharmacology
  • Tetradecanoylphorbol Acetate / pharmacology


  • Gastrins
  • Receptors, Bombesin
  • Receptors, Neurotransmitter
  • Colforsin
  • Substance P
  • Calcimycin
  • Somatostatin
  • spantide
  • Protein Kinase C
  • Adenylyl Cyclases
  • Tetradecanoylphorbol Acetate
  • Bombesin