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. 2013 Dec 13;18(12):15587-99.
doi: 10.3390/molecules181215587.

Antimicrobial and antioxidant activities and effect of 1-hexadecene addition on palmarumycin C2 and C3 yields in liquid culture of endophytic fungus Berkleasmium sp. Dzf12

Affiliations

Antimicrobial and antioxidant activities and effect of 1-hexadecene addition on palmarumycin C2 and C3 yields in liquid culture of endophytic fungus Berkleasmium sp. Dzf12

Yan Mou et al. Molecules. .

Abstract

Two spirobisnaphthalenes, namely palmarumycins C2 and C3, were isolated from cultures of the endophytic fungus Berkleasmium sp. Dzf12 after treatment with 1-hexadecene. After addition of 1-hexadecene at 10% to the medium on day 6 of culture, the maximal yields of palmarumycins C2 and C3 were obtained as 0.40 g/L and 1.19 g/L, which were 40.00 fold and 59.50 fold higher, respectively, in comparison with those of the control (0.01 g/L and 0.02 g/L). The results indicated that addition of 1-hexadecene can be an effective strategy for enhancing the production of palmarumycins C2 and C3 in liquid culture of endophytic fungus Berkleasmium sp. Dzf12. Palmarumycin C3 exhibited stronger antimicrobial and antioxidant activities than palmarumycin C2.

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Figures

Figure 1
Figure 1
HPLC chromatograms of the crude ethyl acetate extracts from endophytic fungus Berkleasmium sp. Dzf12 untreated (A) and treated (B) with 1-hexadecene.
Figure 2
Figure 2
Chemical structures of palmarumycins C3 (1) and C2 (2).
Figure 3
Figure 3
Reducing power of palmarumycins C3 (1) and C2 (2). Each value was expressed as a mean ± SD (n = 3).
Figure 4
Figure 4
Effects of 1-hexadecene and its addition time on mycelia biomass (A), palmarumycin C2 (B) and palmarumycin C3 (C) production in liquid culture of Berkleasmium sp. Dzf12. 1-Hexadecene was applied at 5%, 10% and 15% on days 0, 3, 6, 9, and 12 of culture, respectively. The error bars represent standard deviations from three independent samples. Different letters indicate significant differences among the treatments at p = 0.05 level.

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