Effects of β-glucan on colon anastomotic healing in rats given preoperative irradiation

J Invest Surg. 2014 Jun;27(3):155-62. doi: 10.3109/08941939.2013.865820. Epub 2013 Dec 19.


Background: Radiation therapy is an essential therapeutic modality in the management of a wide variety of tumors. We aimed to investigate the short-term effects of pelvic irradiation on the healing of colon anastomoses and to determine the potential protective effects of β-glucan in this situation.

Material and methods: Sixty Wistar albino rats were randomized into three experimental groups: a control group (n = 20), an irradiation (IR) group (n = 20), and an irradiation+β-glucan (IR+β-glucan) group (n = 20). Only segmental colonic resection and anastomosis were performed on the control group. The IR group underwent the same surgical procedure as the control group 5 days after pelvic irradiation. In the IR+β-glucan group, the same procedure was applied as in the IR group after β-glucan administration. The groups were subdivided into subgroups according to the date of euthanasia (third [n = 10] or seventh [n = 10] postoperative [PO] day), and anastomotic colonic segments were resected to evaluate bursting pressures and biochemical and histopathological parameters.

Results: Bursting pressure values were significantly lower in the IR group (p < .001). Malondialdehyde (MDA) levels were significantly higher in the IR group, whereas β-glucan significantly decreased MDA levels on the third PO day (p < .001). Granulation tissue formation scores were significantly lower in the IR+β-glucan group compared with the control group and the IR group (p < .001).

Conclusions: The results of this study indicate that irradiation has negative effects on the early healing of colon anastomoses. The administration of β-glucan ameliorates these unfavorable effects by altering bursting pressures and biochemical parameters.

MeSH terms

  • Advanced Oxidation Protein Products / metabolism
  • Anastomosis, Surgical
  • Animals
  • Colon / metabolism
  • Colon / pathology
  • Colon / radiation effects
  • Colon / surgery*
  • Drug Evaluation, Preclinical
  • Hydroxyproline / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Radiotherapy / adverse effects*
  • Random Allocation
  • Rats, Wistar
  • Regeneration / drug effects
  • Regeneration / radiation effects*
  • beta-Glucans / pharmacology
  • beta-Glucans / therapeutic use*


  • Advanced Oxidation Protein Products
  • beta-Glucans
  • Malondialdehyde
  • Hydroxyproline