Phospholipid scramblase 1 functionally interacts with angiogenin and regulates angiogenin-enhanced rRNA transcription

Cell Physiol Biochem. 2013;32(6):1695-706. doi: 10.1159/000356604. Epub 2013 Dec 13.


Background: Angiogenin (ANG) can translocate to the target cell nucleus and accumulate in the nucleolus to enhance rRNA transcription, thus promoting cell proliferation. However, the regulation of ANG-enhanced rRNA transcription remains unknown. Previously we identified phospholipid scramblase 1 (PLSCR1) as a potential ANG-interacting protein in yeast two-hybrid screening.

Methods: The interaction was re-confirmed in yeast cells and further verified by in vitro pull down, in vivo co-immunoprecipitation (Co-IP), fluorescent resonance energy transfer (FRET) and immunofluorescence analyses. The rRNA transcription level was determined by real-time quantitative PCR and Northern blot.

Results: PLSCR1 was identified as a novel ANG-interacting protein. Notably, PLSCR1 interacted with ANG in the cell nucleus and regulated rRNA transcription. Furthermore, depletion of cellular ANG expression abolished PLSCR1-enhanced rRNA transcription, which could be rescued by exogenous ANG.

Conclusion: Our data suggest that PLSCR1 positively regulates rRNA transcription through interacting with ANG, thus deepening our understanding on rRNA transcription regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Phospholipid Transfer Proteins / genetics
  • Phospholipid Transfer Proteins / metabolism*
  • Promoter Regions, Genetic
  • Protein Binding
  • RNA Interference
  • RNA, Ribosomal / genetics
  • RNA, Ribosomal / metabolism*
  • RNA, Small Interfering / metabolism
  • Ribonuclease, Pancreatic / antagonists & inhibitors
  • Ribonuclease, Pancreatic / genetics
  • Ribonuclease, Pancreatic / metabolism*
  • Transcription, Genetic
  • Two-Hybrid System Techniques


  • PLSCR1 protein, human
  • Phospholipid Transfer Proteins
  • RNA, Ribosomal
  • RNA, Small Interfering
  • angiogenin
  • Ribonuclease, Pancreatic