Interactome of two diverse RNA granules links mRNA localization to translational repression in neurons

Cell Rep. 2013 Dec 26;5(6):1749-62. doi: 10.1016/j.celrep.2013.11.023. Epub 2013 Dec 19.


Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2. First, neuronal RNA granules are much more heterogeneous than previously anticipated, sharing only a third of the identified proteins. Second, dendritically localized mRNAs, e.g., Arc and CaMKIIα, associate selectively with distinct RNA granules. Third, our work identifies a series of factors with known roles in RNA localization, translational control, and RNA quality control that are likely to keep localized transcripts in a translationally repressed state, often in distinct types of RNPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism
  • Dendrites / metabolism*
  • Hippocampus / cytology
  • Hippocampus / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Protein Binding
  • Protein Biosynthesis*
  • RNA Transport*
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / metabolism
  • Rats
  • Ribonucleoproteins / metabolism


  • Casc3 protein, rat
  • Cytoskeletal Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Stau2 protein, rat
  • activity regulated cytoskeletal-associated protein
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2