The isolation and characterization of RNA coded by the micF gene in Escherichia coli

Nucleic Acids Res. 1987 Mar 11;15(5):2089-101. doi: 10.1093/nar/15.5.2089.

Abstract

A new species of micF RNA, which contains 93 nucleotides (a 4.5S size), was isolated from Escherichia coli. The sequence of the 4.5S micF RNA corresponds to positions G82 through U174 of the micF gene. The 5' terminal end of this smaller micF RNA is triphosphorylated signifying that it is a primary transcript. Its promoter region, which is situated within the greater micF structural gene, has been identified and characterized by lacZ fusion analysis. A 6S micF RNA species, which has a base composition predicted for a transcript from the full length gene has also been detected; however, the 4.5S micF RNA is the predominant species. The work clearly shows by biochemical identification the presence of chromosomally encoded micF RNA.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics*
  • Base Sequence
  • DNA Restriction Enzymes
  • Escherichia coli / genetics*
  • Genes*
  • Genes, Bacterial*
  • Nucleic Acid Conformation
  • Nucleic Acid Hybridization
  • Plasmids
  • RNA / genetics*
  • RNA / isolation & purification
  • RNA, Antisense
  • Transcription, Genetic*

Substances

  • Bacterial Outer Membrane Proteins
  • RNA, Antisense
  • RNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X04888