Efficacy of antibacterial-loaded coating in an in vivo model of acutely highly contaminated implant

Int Orthop. 2014 Jul;38(7):1505-12. doi: 10.1007/s00264-013-2237-2. Epub 2013 Dec 22.


Purpose: The purpose of this study was to test the ability of DAC®, a fast resorbable, antibacterial-loaded hydrogel coating, to prevent acute bacterial colonization in an in vivo model of an intra-operatively highly contaminated implant.

Methods: A histocompatibility study was performed in 10 adult New Zealand rabbits. Then, methicillin-resistant Staph. aureus were inoculated in the femur of 30 adult New Zealand rabbits at the time of intra-medullary nailing; vancomycin-loaded DAC® coated nails were compared to controls regarding local and systemic infection development.

Results: Histocompatibility study showed no detrimental effect of DAC® hydrogel on bone tissue after 12 weeks from implant. After seven days from implant, none of the rabbits receiving vancomycin-loaded DAC® nail showed positive blood cultures, compared to all the controls; vancomycin-loaded DAC® coating was associated with local bacterial load reduction ranging from 72 to 99 %, compared to controls.

Conclusions: Vancomycin-loaded DAC® coating is able to significantly reduce bacterial colonization in an animal model of an intra-operatively highly contaminated implant, without local or general side effect.

MeSH terms

  • Absorbable Implants
  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Coated Materials, Biocompatible
  • Disease Models, Animal
  • Femur / microbiology*
  • Hydrogels
  • Male
  • Prostheses and Implants
  • Prosthesis-Related Infections / drug therapy*
  • Prosthesis-Related Infections / surgery
  • Rabbits
  • Staphylococcal Infections / drug therapy*
  • Staphylococcus aureus*
  • Vancomycin / administration & dosage*


  • Anti-Bacterial Agents
  • Coated Materials, Biocompatible
  • Hydrogels
  • Vancomycin