Dehydroalanine-based diubiquitin activity probes

Org Lett. 2014 Jan 17;16(2):540-3. doi: 10.1021/ol403416w. Epub 2013 Dec 24.


A strategy for the synthesis of dehydroalanine based diubiquitin activity probes is described. The site-specific introduction of dehydroalanine was achieved from diubiquitin bearing Cys residue near the scissile bond between two ubiquitins linked through Lys48, Lys63 or in a head to tail fashion. The probes were characterized for their activities with various deubiquitinases, which open new opportunities in studying deubiquitinases in various settings.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / analogs & derivatives*
  • Alanine / chemistry
  • Amino Acid Sequence
  • Humans
  • Lysine / chemistry
  • Models, Molecular
  • Molecular Structure
  • Protein Conformation
  • Ubiquitin-Specific Proteases / metabolism*
  • Ubiquitins / metabolism*


  • UBD protein, human
  • Ubiquitins
  • dehydroalanine
  • Ubiquitin-Specific Proteases
  • Lysine
  • Alanine