Antioxidant activity and protective effects of peptide lunasin against oxidative stress in intestinal Caco-2 cells

Food Chem Toxicol. 2014 Mar;65:155-61. doi: 10.1016/j.fct.2013.12.021. Epub 2013 Dec 21.


Oxidative stress is one of the most critical factors implicated in many disorders of the gastrointestinal tract, such as inflammatory bowel diseases and colon cancer. Lunasin is a seed peptide known by its properties against cancer and cardiovascular diseases. The present study investigated the in vitro antioxidant activity of this peptide that demonstrated to be a potent scavenger of peroxyl and superoxide radicals. In addition, the protective role of lunasin on cell viability and antioxidant defenses of human Caco-2 cells challenged by hydrogen peroxide and tert-butylhydroperoxide was evaluated. This peptide remained partially intact during incubation time with cells, preventing of the oxidative damage induced by both chemical agents. The direct antioxidant action of lunasin (within the physiological range of concentrations) on enterocytes exposed to oxidizing species makes this peptide a promising agent to preserve the integrity of intestinal mucosa against oxidative damage related diseases.

Keywords: Antioxidant; Intestinal health; Oxidative stress; Peptide lunasin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Caco-2 Cells
  • Humans
  • Intestines / cytology
  • Intestines / drug effects*
  • Oxidative Stress / drug effects*
  • Plant Proteins / pharmacology*
  • Reactive Oxygen Species / metabolism


  • Antioxidants
  • Plant Proteins
  • Reactive Oxygen Species