Abstract
A total of 166 faecal specimens from diarrheic cattle were collected in China for detection of bovine kobuvirus (BKV) by reverse transcription PCR (RT-PCR) targeting the region a portion of the 3D nonstructural protein, with an amplicon size of 631 bp. The RNA corresponding to the BKV 3D region was detected in 34.9 % of faecal samples (58/166) in four major dairy-cattle-production areas in China, and sequence analysis based on the partial 3D sequences (35/58) indicated that the Chinese BKVs shared 88.9-96.2 % nucleotide sequence identity to BKV reference strains. Further phylogenetic analysis based on the complete VP1-encoding sequences (17/35) revealed that the Chinese BKVs shared 81-83.4 % nucleotide sequence identity to the U-1 strain, and these Chinese BKV strains, together with the U-1 strain, are apparently divided into four lineages, representing four genotypes of BKV, designated as A, B, C and D. Our results show that BKV infection is widely distributed, with high genetic diversity in China.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cattle Diseases / virology*
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China / epidemiology
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Cluster Analysis
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Diarrhea / veterinary*
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Diarrhea / virology
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Genetic Variation*
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Kobuvirus / classification*
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Kobuvirus / genetics
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Kobuvirus / isolation & purification*
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Molecular Epidemiology
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Molecular Sequence Data
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Phylogeny
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Picornaviridae Infections / veterinary*
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Picornaviridae Infections / virology
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Prevalence
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology
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Viral Nonstructural Proteins / genetics
Substances
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RNA, Viral
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Viral Nonstructural Proteins
Associated data
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GENBANK/KF728676
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GENBANK/KF728677
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GENBANK/KF728678
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GENBANK/KF728679
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GENBANK/KF728680
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