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. 2014;58(3):1793-6.
doi: 10.1128/AAC.02024-13. Epub 2013 Dec 23.

Green Fluorescent Diamidines as Diagnostic Probes for Trypanosomes

Free PMC article

Green Fluorescent Diamidines as Diagnostic Probes for Trypanosomes

Federica Giordani et al. Antimicrob Agents Chemother. .
Free PMC article


Light-emitting diode (LED) fluorescence microscopy offers potential benefits in the diagnosis of human African trypanosomiasis and in other aspects of diseases management, such as detection of drug-resistant strains. To advance such approaches, reliable and specific fluorescent markers to stain parasites in human fluids are needed. Here we describe a series of novel green fluorescent diamidines and their suitability as probes with which to stain trypanosomes.


(A) Fluorescence images of infected rat blood incubated ex vivo with the five green fluorescent diamidines (note that the parasite position can change between the bright-field and corresponding fluorescence micrographs), 40× objective. (B) In vitro trypanosomes treated with DB1680 and DB1692 (50 μM, 1 h, 37°C), 100× objective; 4′,6-diamidino-2-phenylindole (DAPI) (20 μM) was used as a DNA counterstain. Asterisk, kinetoplast; arrow, cytoplasmic corpuscles, possibly acidocalcisomes; arrowhead, nucleus; hash mark, possibly lysosome. Bar, 10 μm.

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