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. 2014;58(3):1793-6.
doi: 10.1128/AAC.02024-13. Epub 2013 Dec 23.

Green Fluorescent Diamidines as Diagnostic Probes for Trypanosomes

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Free PMC article

Green Fluorescent Diamidines as Diagnostic Probes for Trypanosomes

Federica Giordani et al. Antimicrob Agents Chemother. .
Free PMC article

Abstract

Light-emitting diode (LED) fluorescence microscopy offers potential benefits in the diagnosis of human African trypanosomiasis and in other aspects of diseases management, such as detection of drug-resistant strains. To advance such approaches, reliable and specific fluorescent markers to stain parasites in human fluids are needed. Here we describe a series of novel green fluorescent diamidines and their suitability as probes with which to stain trypanosomes.

Figures

FIG 1
FIG 1
(A) Fluorescence images of infected rat blood incubated ex vivo with the five green fluorescent diamidines (note that the parasite position can change between the bright-field and corresponding fluorescence micrographs), 40× objective. (B) In vitro trypanosomes treated with DB1680 and DB1692 (50 μM, 1 h, 37°C), 100× objective; 4′,6-diamidino-2-phenylindole (DAPI) (20 μM) was used as a DNA counterstain. Asterisk, kinetoplast; arrow, cytoplasmic corpuscles, possibly acidocalcisomes; arrowhead, nucleus; hash mark, possibly lysosome. Bar, 10 μm.

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References

    1. Barrett MP, Burchmore RJ, Stich A, Lazzari JO, Frasch AC, Cazzulo JJ, Krishna S. 2003. The trypanosomiases. Lancet 362:1469–1480. 10.1016/S0140-6736(03)14694-6 - DOI - PubMed
    1. Chappuis F, Loutan L, Simarro P, Lejon V, Buscher P. 2005. Options for field diagnosis of human African trypanosomiasis. Clin. Microbiol. Rev. 18:133–146. 10.1128/CMR.18.1.133-146.2005 - DOI - PMC - PubMed
    1. Hanscheid T. 2008. The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae. Trans. R. Soc. Trop. Med. Hyg. 102:520–521. 10.1016/j.trstmh.2008.02.020 - DOI - PubMed
    1. Jones D, Nyalwidhe J, Tetley L, Barrett MP. 2007. McArthur revisited: fluorescence microscopes for field diagnostics. Trends Parasitol. 23:468–469. 10.1016/j.pt.2007.08.003 - DOI - PubMed
    1. Minion J, Sohn H, Pai M. 2009. Light-emitting diode technologies for TB diagnosis: what is on the market? Expert. Rev. Med. Devices 6:341–345. 10.1586/erd.09.26 - DOI - PubMed

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