Stimulation of macrophage tumoricidal activity by the growth and differentiation factor CSF-1

Cell Immunol. 1987 Apr 1;105(2):270-9. doi: 10.1016/0008-8749(87)90076-1.

Abstract

The effect of the macrophage growth and differentiation factor CSF-1 on the tumoricidal capacity of murine peritoneal exudate macrophages was investigated. Pretreatment of peptone-elicited macrophages 1 day with 300-1200 U/ml CSF-1 induced moderate killing and greatly stimulated lymphokine (LK)-induced killing of [3H]thymidine-labeled TU5 sarcoma cells to levels above that seen with fresh macrophages. Further addition of CSF-1 at Day 1 at the time of the tumor lysis assay promoted moderate increases in spontaneous and LK-induced activity. CSF-1 did not stimulate freshly harvested exudate macrophages to lyse TU5 targets in the presence or absence of lymphokine (LK) activators. Lipopolysaccharide (LPS) at 0.1-1000 ng/ml did not stimulate cytotoxicity, and the low endotoxin content and the use of polymyxin B and C3H/HeJ mice excluded a role for LPS in these experiments. Incubation of the macrophages with IFN and the myeloid growth factors IL-3 and GM-CSF did not stimulate tumoricidal activity. CSF-1 has been proposed as a therapeutic agent to restore myeloid cell numbers in induced (cancer chemotherapy, bone marrow transplantation, etc.) and natural aplastic anemias. These studies show that CSF-1 also may be useful in combination with LK activators to promote resistance to cancer in mature mononuclear cells. CSF-1 may have similar effects in LK-activated macrophages to enhance resistance to infectious diseases.

MeSH terms

  • Animals
  • Cell Line
  • Colony-Stimulating Factors / pharmacology*
  • Cytotoxicity, Immunologic / drug effects*
  • Female
  • Interferons / pharmacology
  • Lipopolysaccharides / immunology
  • Lymphokines / physiology
  • Macrophage Activation / drug effects*
  • Mice
  • Mice, Inbred Strains
  • Peritoneum / cytology
  • Sarcoma, Experimental / immunology

Substances

  • Colony-Stimulating Factors
  • Lipopolysaccharides
  • Lymphokines
  • Interferons