Preparation and antibacterial activity of oligosaccharides derived from dandelion

Int J Biol Macromol. 2014 Mar;64:392-4. doi: 10.1016/j.ijbiomac.2013.12.031. Epub 2013 Dec 22.


In this study, we prepared oligosaccharides from dandelion (Taraxacum officinale) by hydrolysis with hydrogen peroxide (H2O2) and investigated their antibacterial activity. The optimum hydrolysis conditions, as determined using the response surface methodology, were as follows: reaction time, 5.12h; reaction temperature, 65.53 °C and H2O2 concentration, 3.16%. Under these conditions, the maximum yield of the oligosaccharides reached 25.43%. The sugar content in the sample was 96.8%, and the average degree of polymerisation was approximately 9. The oligosaccharides showed high antibacterial activity against Escherichia coli, Bacillus subtilis and Staphylococcus aureus, indicating that dandelion-derived oligosaccharides have the potential to be used as antibacterial agents.

Keywords: Antibacterial activities; Dandelion-derived oligosaccharides; Hydrogen peroxide.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Bacillus subtilis / drug effects
  • Escherichia coli / drug effects
  • Hydrogen Peroxide / chemistry
  • Hydrogen-Ion Concentration
  • Microbial Sensitivity Tests
  • Oligosaccharides / chemistry
  • Oligosaccharides / pharmacology*
  • Staphylococcus aureus / drug effects
  • Taraxacum / chemistry*
  • Temperature


  • Anti-Bacterial Agents
  • Oligosaccharides
  • Hydrogen Peroxide