Expansion and functional activity of Ly-1+ B cells upon transfer of peritoneal cells into allotype-congenic, newborn mice

Eur J Immunol. 1987 Apr;17(4):521-8. doi: 10.1002/eji.1830170414.

Abstract

Transfer of peritoneal cells from adult donors into newborn, allotype-congenic mice led to colonization of the recipient mice by donor-derived B lymphocytes expressing the Ly-1 surface marker (Ly-1 B cells). These cells not only persisted in the recipient mice for at least 5 months, but also increased in number. In contrast, bone marrow-derived stem cells did not or scarcely give rise to B cells after intraperitoneal injection into congenic newborn recipients under the same experimental conditions. Approximately half of the IgM in the serum of peritoneal cell-recipients was produced by donor-derived Ly-1 B cells, suggesting that high levels of serum IgM in a normal mouse are produced by this B cell subpopulation. The transferred Ly-1 B cells were able to respond in a normal fashion to alpha (1----3)dextran, but they did not participate in thymus-dependent and -independent (TI II) immune responses to the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). In neither the immune response to alpha (1----3)dextran nor that to NP were we able to detect an influence of the transferred Ly-1 B cells on the selection of the idiotypic repertoire in the recipient mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn / immunology
  • Antigens, Ly / analysis*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / transplantation
  • Cell Division
  • Dextrans / pharmacology
  • Immunoglobulin M / biosynthesis
  • Immunoglobulin lambda-Chains / biosynthesis
  • Mice
  • Nitrophenols / immunology
  • Peritoneal Cavity / cytology
  • Phenylacetates
  • Spleen / cytology

Substances

  • Antigens, Ly
  • Dextrans
  • Immunoglobulin M
  • Immunoglobulin lambda-Chains
  • Nitrophenols
  • Phenylacetates
  • 4-hydroxy-5-nitrophenyl acetic acid