Characterization of antidiabetic and antihypertensive properties of canary seed (Phalaris canariensis L.) peptides

J Agric Food Chem. 2014 Jan 15;62(2):427-33. doi: 10.1021/jf404539y. Epub 2014 Jan 6.


Canary grass is used as traditional food for diabetes and hypertension treatment. The aim of this work is to characterize the biological activity of encrypted peptides released after gastrointestinal digestion of canary seed proteins. Canary peptides showed 43.5% inhibition of dipeptidyl peptidase IV (DPPIV) and 73.5% inhibition of angiotensin-converting enzyme (ACE) activity. An isolated perfused rat heart system was used to evaluate the canary seed vasoactive effect. Nitric oxide (NO), a major vasodilator agent, was evaluated in the venous effluent from isolated perfused rat heart. Canary seed peptides (1 μg/mL) were able to induce the production of NO (12.24 μM) in amounts similar to those induced by captopril (CPT) and bradykinin (BK). These results show that encrypted peptides in canary seed have inhibitory activity against DPPIV and ACE, enzymes that are targets for diabetes and hypertension treatments.

Keywords: angiotensin-converting enzyme; canary seed; dipeptidyl peptidase IV; encrypted peptides; nitric oxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / pharmacology
  • Animals
  • Antihypertensive Agents / pharmacology*
  • Bradykinin
  • Captopril
  • Coronary Vessels / drug effects
  • Digestion
  • Hypoglycemic Agents / pharmacology*
  • Male
  • Myocardium / metabolism
  • Nitric Oxide / analysis
  • Nitric Oxide / biosynthesis
  • Peptides / isolation & purification
  • Peptides / metabolism
  • Peptides / pharmacology*
  • Peptidyl-Dipeptidase A / metabolism
  • Phalaris / chemistry*
  • Rats
  • Rats, Wistar
  • Seed Storage Proteins / chemistry
  • Seed Storage Proteins / isolation & purification
  • Seed Storage Proteins / metabolism
  • Seeds / chemistry*
  • Vasodilation / drug effects
  • Vasodilator Agents / pharmacology


  • Angiotensin-Converting Enzyme Inhibitors
  • Antihypertensive Agents
  • Hypoglycemic Agents
  • Peptides
  • Seed Storage Proteins
  • Vasodilator Agents
  • Nitric Oxide
  • Captopril
  • Peptidyl-Dipeptidase A
  • Bradykinin