Ultrasensitive IL-6 electrochemical immunosensor based on Au nanoparticles-graphene-silica biointerface

Guangfeng Wang; Xiuping He; Ling Chen; Yanhong Zhu; Xiaojun Zhang

doi:10.1016/j.colsurfb.2013.11.015

Ultrasensitive IL-6 electrochemical immunosensor based on Au nanoparticles-graphene-silica biointerface

Colloids Surf B Biointerfaces. 2014 Apr 1:116:714-9. doi: 10.1016/j.colsurfb.2013.11.015. Epub 2013 Nov 20.

Authors

Guangfeng Wang¹, Xiuping He², Ling Chen², Yanhong Zhu², Xiaojun Zhang³

Affiliations

¹ College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China; Anhui Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Hefei 230009, PR China.
² College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China.
³ College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China. Electronic address: xjzhang@mail.ahnu.edu.cn.

PMID: 24370115
DOI: 10.1016/j.colsurfb.2013.11.015

Abstract

An Interleukin-6 (IL-6) electrochemical immunosensor was fabricated based on the Au nanoparticles (AuNP)-graphene-silica sol-gel as immobilization biointerface and AuNP-polydopamine (PDA)@carbon nanotubes (CNT) as the label of HRP-bound antibodies. The AuNP-graphene-silica sol-gel film was prepared in situ and modified on the ITO electrode, providing a stable network for the immobilization of antibody and exhibiting a dynamic working range of 1-40 pg/mL with a low detection limit of 0.3 pg/mL IL-6 (at 3s). The results of serum samples with the sensor received an acceptable agreement with the ELISA method. Importantly, this method provided a promising ultrasensitive assay strategy for clinical applications.

Keywords: Electrochemical immunosensor; Graphene; Interleukin-6; Polydopamine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrochemical Techniques*
Enzyme-Linked Immunosorbent Assay
Gold / chemistry*
Graphite / chemistry*
Immunoassay*
Interleukin-6 / analysis*
Metal Nanoparticles / chemistry*
Particle Size
Silicon Dioxide / chemistry*
Surface Properties

Substances

Interleukin-6
Gold
Silicon Dioxide
Graphite