Evaluation of two commercial real-time PCR assays for detection of Mycoplasma genitalium in urogenital specimens

J Clin Microbiol. 2014 Mar;52(3):971-3. doi: 10.1128/JCM.02567-13. Epub 2013 Dec 26.

Abstract

The performance of two commercial real-time PCR kits for the detection of Mycoplasma genitalium was evaluated in comparison to an in-house real-time PCR assay. Concordances of 96% and 93% were found for the TIB MOLBIOL and the Diagenode assays, respectively, compared to the results of the in-house assay.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / methods*
  • Bodily Secretions / microbiology
  • Female
  • Female Urogenital Diseases / diagnosis*
  • Female Urogenital Diseases / microbiology
  • Humans
  • Male
  • Male Urogenital Diseases / diagnosis*
  • Male Urogenital Diseases / microbiology
  • Molecular Diagnostic Techniques / methods*
  • Mycoplasma Infections / diagnosis*
  • Mycoplasma Infections / microbiology
  • Mycoplasma genitalium / isolation & purification*
  • Real-Time Polymerase Chain Reaction / methods*
  • Urine / microbiology