High-performance liquid chromatography purification of chemically modified RNA aptamers

Chi-Yen Lin; Zhen Huang; William Jaremko; Li Niu

doi:10.1016/j.ab.2013.12.022

High-performance liquid chromatography purification of chemically modified RNA aptamers

Anal Biochem. 2014 Mar 15:449:106-8. doi: 10.1016/j.ab.2013.12.022. Epub 2013 Dec 25.

Authors

Chi-Yen Lin¹, Zhen Huang¹, William Jaremko¹, Li Niu²

Affiliations

¹ Department of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
² Department of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA. Electronic address: lniu@albany.edu.

Abstract

2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many known RNA aptamers of therapeutic values, our protocol should be generally useful.

Keywords: Chemically modified RNA; HPLC; RNA purification and separation.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry
Aptamers, Nucleotide / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Chromatography, Reverse-Phase / methods
Halogenation
Ions / chemistry

Substances

Aptamers, Nucleotide
Ions

Grants and funding

R01 NS060812/NS/NINDS NIH HHS/United States