Large-scale mapping of transposable element insertion sites using digital encoding of sample identity

Genetics. 2014 Mar;196(3):615-23. doi: 10.1534/genetics.113.159483. Epub 2013 Dec 27.


Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools.

Keywords: Drosophila melanogaster; mapping; next-generation sequencing; transposable elements.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosome Mapping
  • DNA Transposable Elements*
  • Drosophila melanogaster / genetics*
  • Genome, Insect
  • Genomics / methods*
  • High-Throughput Nucleotide Sequencing
  • Sequence Analysis, DNA


  • DNA Transposable Elements