Centromere localization for Bighead Carp (Aristichthys nobilis) through half-tetrad analysis in diploid gynogenetic families

PLoS One. 2013 Dec 20;8(12):e82950. doi: 10.1371/journal.pone.0082950. eCollection 2013.

Abstract

Gene-centromere (G-C) mapping provides insights into structural and behavioural properties of chromosomes. In this study, G-C mapping using microsatellite markers and meiogynogenetic (meiotic gynogenetic) families were performed in bighead carp (Aristichthys nobilis, 2N = 48), which belongs to Cyprinidae. A total of 218 microsatellites were selected across 24 linkage groups (LGs) of a recently well-defined genetic linkage map for bighead carp, with 151 being heterozygous in at least one of six dams in diploid meiogynogenetic families. After tests for Mendelian segregation in two diploid control families, 103 microsatellites were used for G-C distance calculation in 383 gynogens. The second division segregation frequency (y) was computed through half-tetrad analyses, and the values ranged from 0 to 0.97 (mean 0.40). High G-C recombination frequencies (over 0.667) were observed in 18 (17.5%) of the loci examined, which revealed a low level of chiasma interferences compared with other fishes studied previously. Distribution of G-C distances across LGs ranged from 0 cM to 48.5 cM (mean 20 cM) under the assumption of complete interference. All 24 centromeres were localized according to their closest-related microsatellites at 95% confident intervals. The average distance between centromeres and their closest-linked markers was 6.1 cM with 15 out of 24 LGs having a distance below 5 cM. Based on the centromere positions in this study, we proposed a formula of 24 m/sm+24 t/st chromosomes with 92 arms for bighead carp, which was mostly in accordance with a previously reported karyotype for bighead carp (24 m/sm+24 st). These results of centromere localization provide a basic framework and important resources for genetics and comparative genomics studies in bighead carp and its closely-related cyprinid species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carps / genetics*
  • Centromere*
  • Chromosome Mapping
  • Diploidy*
  • Female
  • Genetic Linkage
  • Genetic Loci
  • Genotype
  • Heterozygote
  • Inheritance Patterns
  • Karyotype*
  • Microsatellite Repeats
  • Recombination, Genetic

Grant support

This study was financially supported by research grants from the NSFC (31272647), MOA (200903045, 2011-G12) and FEBL (2011FBZ20) of China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.