Ameliorating effect and potential mechanism of Rehmannia glutinosa oligosaccharides on the impaired glucose metabolism in chronic stress rats fed with high-fat diet

Phytomedicine. 2014 Apr 15;21(5):607-14. doi: 10.1016/j.phymed.2013.11.008. Epub 2013 Dec 28.


The aim of this study was to determine whether the Rehmannia glutinosa oligosaccharides (ROS) ameliorate the impaired glucose metabolism and the potential mechanism in chronic stress rats fed with high-fat diet. The rats were fed by a high-fat diet and simultaneously stimulated by chronic stress over 5 weeks. Body weight, fasting plasma glucose, intraperitoneal glucose tolerance test (IPGTT), plasma lipids, gluconeogenesis test (GGT), glycogen content, and corticosterone, insulin and leptin levels were measured. The results showed that ROS administration (100, 200 mg/kg, i.g.) for 5 weeks exerted the effects of increasing the organ weights of thymus and spleen, lowering the fasting plasma glucose level, improving impaired glucose tolerance, increasing the contents of liver and muscle glycogen, decreasing the gluconeogenesis ability, plasma-free fatty acid's level, as well as plasma triglyceride and total cholesterol levels in chronic stress and high-fat fed rats, especially in the group of 200mg/kg; while the plasma corticosterone level was decreased, and plasma leptin level was increased. These results suggest that ROS exert an ameliorating effect of impaired glucose metabolism in chronic stress rats fed with high-fat diet, and the potential mechanism may be mediated through rebuilding the glucose homeostasis in the neuroendocrine immuno-modulation (NIM) network through multilinks and multitargets.

Keywords: Chronic stress; HPA axis; High-fat diet; Impaired glucose metabolism; Rehmannia glutinosa oligosaccharides (ROS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • Corticosterone / blood
  • Diet, High-Fat / adverse effects
  • Drug Evaluation, Preclinical
  • Glucose Metabolism Disorders / drug therapy*
  • Glucose Metabolism Disorders / etiology
  • Glucose Metabolism Disorders / metabolism
  • Glucose Tolerance Test
  • Glycogen / metabolism
  • Insulin / blood
  • Leptin / blood
  • Lipids / blood
  • Liver / metabolism
  • Male
  • Muscles / metabolism
  • Oligosaccharides / pharmacology
  • Oligosaccharides / therapeutic use*
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Plants, Medicinal
  • Random Allocation
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism*
  • Rehmannia*
  • Stress, Psychological / complications
  • Stress, Psychological / metabolism


  • Blood Glucose
  • Insulin
  • Leptin
  • Lipids
  • Oligosaccharides
  • Plant Extracts
  • Reactive Oxygen Species
  • Glycogen
  • Corticosterone