[Role of Dlx1 natural antisense transcript in mice brain development]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2013 Dec;35(6):607-10. doi: 10.3881/j.issn.1000-503X.2013.06.004.
[Article in Chinese]


Objective: To explore the role of Dlx1as, a natural antisense transcript, in mice brain development.

Methods: Based on the bioinformatic and molecular biological findings, we confirmed whether the Dlx1as has a poly-A tail. The temporal expressions of Dlx1as and Dlx1 mRNA were determined by quantitative real-time PCR and the spatial expressions of Dlx1as and Dlx1 mRNA were compared by using in situ hybridization.

Results: Dlx1as had a poly-A signal. Dlx1as's expression was at a high level through a period of time in the embryo development, which was similar with Dlx1 mRNA in temporal but different in spatial.

Conclusions: Dlx1as is highly expressed in a certain period during mice brain development, which spatially supplements the mRNA expression of D1x1. Therefore, it is supposed that Dlx1as influences the mice brain development by regulating Dlx1 mRNA.

MeSH terms

  • Animals
  • Brain / growth & development*
  • Brain / metabolism
  • Homeodomain Proteins / metabolism*
  • In Situ Hybridization
  • Mice
  • RNA, Messenger
  • Real-Time Polymerase Chain Reaction
  • Transcription Factors / metabolism*


  • Distal-less homeobox proteins
  • Homeodomain Proteins
  • RNA, Messenger
  • Transcription Factors