Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

Ryan A Colyer; Giuseppe Scalia; Federica A Villa; Fabrizio Guerrieri; Simone Tisa; Franco Zappa; Sergio Cova; Shimon Weiss; Xavier Michalet

doi:10.1117/12.874238

Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

Proc SPIE Int Soc Opt Eng. 2011 Feb 28:7905:10.1117/12.874238. doi: 10.1117/12.874238.

Authors

Ryan A Colyer¹, Giuseppe Scalia¹, Federica A Villa², Fabrizio Guerrieri², Simone Tisa³, Franco Zappa², Sergio Cova², Shimon Weiss¹, Xavier Michalet¹

Affiliations

¹ Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.
² Dipartimento di Elettronica ed Informazione, Politecnico di Milano, Milano, Italy.
³ Micro Photon Devices, Bolzano, Italy.

Abstract

Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness, conformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with good statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition speed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in throughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS 1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.

Keywords: CMOS; FCS; LCOS; SPAD array; fluorescence; high-throughput; photon counting; single-molecule.

Abstract

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