Site-directed cleavage of RNA

Nucleic Acids Res. 1987 Jun 11;15(11):4403-15. doi: 10.1093/nar/15.11.4403.

Abstract

Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.

MeSH terms

  • Base Sequence
  • Endoribonucleases*
  • Hydrogen Bonding
  • Nucleic Acid Conformation
  • Oligoribonucleotides*
  • Q beta Replicase / metabolism
  • RNA / analysis*
  • RNA, Double-Stranded / metabolism
  • Ribonuclease H

Substances

  • Oligoribonucleotides
  • RNA, Double-Stranded
  • RNA
  • Q beta Replicase
  • Endoribonucleases
  • Ribonuclease H