Blockage of chloride channels by HEPES buffer

Proc R Soc Lond B Biol Sci. 1987 Feb 23;230(1258):93-100. doi: 10.1098/rspb.1987.0011.

Abstract

Chloride channels of neurons of Drosophila are blocked when the cytoplasmic side of the membrane is exposed to the commonly used buffering agents 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid (HEPES) and 4-morpholinepropanesulphonic acid (MOPS). In the presence of these compounds, chloride channels appear to function as a complex of multiple protochannels.

MeSH terms

  • Animals
  • Chlorides / metabolism*
  • Drosophila
  • HEPES / pharmacology*
  • Ion Channels / drug effects
  • Ion Channels / physiology*
  • Membrane Potentials / drug effects
  • Neural Conduction / drug effects
  • Neurons / physiology
  • Piperazines / pharmacology*

Substances

  • Chlorides
  • Ion Channels
  • Piperazines
  • HEPES