Resolvin D1 attenuates lipopolysaccharide induced acute lung injury through CXCL-12/CXCR4 pathway

J Surg Res. 2014 May 1;188(1):213-21. doi: 10.1016/j.jss.2013.11.1107. Epub 2013 Dec 6.


Background: The recruitment of neutrophils plays an important role in the progress of acute lung injury (ALI). Excessive neutrophils released from bone marrow accumulate in lung, release proinflammatory factors, and cause tissue damage. CXCL-12/CXCR4 is an important signaling pathway, which regulates the migration of bone marrow hematopoietic cells out of bone marrow and involves in neutrophil accumulation and retention in the inflammatory site. Resolvin D1 (RvD1) is a kind of lipid mediators, which can alleviate many inflammatory diseases. We hypothesized that RvD1 can alleviate lipopolysaccharide (LPS)-induced ALI through regulating CXCL-12/CXCR4 pathway.

Methods: We randomized mice into five groups: control group, RvD1 group, LPS group, LPS plus RvD1 group, and LPS plus AMD3100 group. ALI was established by intratracheal instillation of LPS. After 24 and 72 h, mice were sacrificed, and lung tissues were harvested for histologic analysis, wet-to-dry ratio, myeloperoxidase activity, and CXCL-12 expression. Bronchoalveolar fluid was collected for protein analysis, cytokines assay, and flow cytometry analysis.

Results: Histologic findings as well as wet-to-dry ratio, protein concentration, cytokines assay, neutrophil number, and myeloperoxidase activity confirmed that RvD1 and AMD3100 alleviated LPS-induced ALI. RvD1 decreased CXCL-12 messenger RNA expression in lung. However, RvD1 promoted CXCR4 expression in neutrophils in the initial stage of inflammation and reduced its level in the later stage.

Conclusions: RvD1 protects LPS-induced ALI partially through regulating CXCL-12/CXCR4 pathway.

Keywords: Acute lung injury; Inflammation; Neutrophil; Resolvin D1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / immunology
  • Acute Lung Injury / metabolism
  • Acute Lung Injury / pathology
  • Acute Lung Injury / prevention & control*
  • Animals
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / cytology
  • Chemokine CXCL12 / metabolism*
  • Docosahexaenoic Acids / pharmacology
  • Docosahexaenoic Acids / therapeutic use*
  • Drug Evaluation, Preclinical
  • Interleukin-1beta / metabolism
  • Lipopolysaccharides
  • Lung / metabolism
  • Lung / pathology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Peroxidase / metabolism
  • Random Allocation
  • Receptors, CXCR4 / metabolism*
  • Signal Transduction
  • Tumor Necrosis Factor-alpha / metabolism


  • CXCR4 protein, mouse
  • Chemokine CXCL12
  • Cxcl12 protein, mouse
  • IL1B protein, mouse
  • Interleukin-1beta
  • Lipopolysaccharides
  • Receptors, CXCR4
  • Tumor Necrosis Factor-alpha
  • resolvin D1
  • Docosahexaenoic Acids
  • Peroxidase