Abstract
A recombinant Huh7-PPRE-Luc cell line, carrying a peroxisome proliferator response element (PPRE)-driven luciferase gene, is an efficient tool for evaluation of potential peroxisome proliferator-activated receptor (PPAR) agonists. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of PPAR agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Here, the bioassay is fitted into a 96-well plate format for high-throughput screening purpose.
Keywords:
High-throughput screening; Luciferase reporter gene; PPAR agonists; PPRE promoter assay; RXR agonists.
© 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Cell Line
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Clone Cells
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Drug Discovery / methods*
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Endocrine Disruptors / pharmacology
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Genes, Reporter / drug effects
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Green Fluorescent Proteins / metabolism
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High-Throughput Screening Assays
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Humans
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Luciferases / genetics
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Luciferases / metabolism
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Peroxisome Proliferator-Activated Receptors / agonists*
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Peroxisome Proliferator-Activated Receptors / genetics
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Peroxisome Proliferator-Activated Receptors / metabolism
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Peroxisome Proliferators / pharmacology*
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Promoter Regions, Genetic / drug effects
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Response Elements / drug effects*
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Thymidine Kinase / genetics
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Thymidine Kinase / metabolism
Substances
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Endocrine Disruptors
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Peroxisome Proliferator-Activated Receptors
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Peroxisome Proliferators
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Recombinant Fusion Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Luciferases
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Thymidine Kinase