A recombinant PPRE-driven luciferase bioassay for identification of potential PPAR agonists

Vitam Horm. 2014:94:427-35. doi: 10.1016/B978-0-12-800095-3.00015-8.

Abstract

A recombinant Huh7-PPRE-Luc cell line, carrying a peroxisome proliferator response element (PPRE)-driven luciferase gene, is an efficient tool for evaluation of potential peroxisome proliferator-activated receptor (PPAR) agonists. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of PPAR agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Here, the bioassay is fitted into a 96-well plate format for high-throughput screening purpose.

Keywords: High-throughput screening; Luciferase reporter gene; PPAR agonists; PPRE promoter assay; RXR agonists.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Line
  • Clone Cells
  • Drug Discovery / methods*
  • Endocrine Disruptors / pharmacology
  • Genes, Reporter / drug effects
  • Green Fluorescent Proteins / metabolism
  • High-Throughput Screening Assays
  • Humans
  • Luciferases / genetics
  • Luciferases / metabolism
  • Peroxisome Proliferator-Activated Receptors / agonists*
  • Peroxisome Proliferator-Activated Receptors / genetics
  • Peroxisome Proliferator-Activated Receptors / metabolism
  • Peroxisome Proliferators / pharmacology*
  • Promoter Regions, Genetic / drug effects
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Response Elements / drug effects*
  • Thymidine Kinase / genetics
  • Thymidine Kinase / metabolism

Substances

  • Endocrine Disruptors
  • Peroxisome Proliferator-Activated Receptors
  • Peroxisome Proliferators
  • Recombinant Fusion Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Luciferases
  • Thymidine Kinase