Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system

Adam H Kramer; Julia Joos-Vandewalle; Adrienne L Edkins; Carminita L Frost; Earl Prinsloo

doi:10.1016/j.bbrc.2013.12.123

Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system

Biochem Biophys Res Commun. 2014 Jan 24;443(4):1245-50. doi: 10.1016/j.bbrc.2013.12.123. Epub 2014 Jan 3.

Authors

Adam H Kramer¹, Julia Joos-Vandewalle¹, Adrienne L Edkins¹, Carminita L Frost², Earl Prinsloo³

Affiliations

¹ Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa.
² Department of Biochemistry and Microbiology, PO Box 77000, Nelson Mandela Metropolitan University, Port Elizabeth 6031, South Africa.
³ Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa. Electronic address: e.prinsloo@ru.ac.za.

PMID: 24388983
DOI: 10.1016/j.bbrc.2013.12.123

Abstract

Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.

Keywords: 3T3-L1 cells; Adipogenesis; Impedance; Real-time analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1-Methyl-3-isobutylxanthine / pharmacology
3T3-L1 Cells
Adipocytes / cytology*
Adipocytes / drug effects
Adipogenesis / drug effects
Animals
Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Cell Differentiation* / drug effects
Computer Systems
Cytological Techniques / instrumentation*
Dexamethasone / pharmacology
Electric Impedance
Insulin / pharmacology
Mice
Rosiglitazone
Thiazolidinediones / pharmacology

Substances

Insulin
Thiazolidinediones
Rosiglitazone
Dexamethasone
1-Methyl-3-isobutylxanthine