Look Ma, no PCNA: how DNA polymerase ε synthesizes long stretches of DNA without a processivity factor

Karl E Zahn; Sylvie Doublié

doi:10.1038/nsmb.2749

Look Ma, no PCNA: how DNA polymerase ε synthesizes long stretches of DNA without a processivity factor

Nat Struct Mol Biol. 2014 Jan;21(1):12-4. doi: 10.1038/nsmb.2749.

Authors

Karl E Zahn¹, Sylvie Doublié¹

Affiliation

¹ Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA.

Abstract

The long-awaited crystal structure of Saccharomyces cerevisiae DNA polymerase ε reveals a unique domain never before observed in family B DNA polymerases. This novel domain endows polymerase ε with a capacity for highly processive DNA synthesis.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

DNA / biosynthesis*
DNA Polymerase II / chemistry
DNA Polymerase II / metabolism*
Models, Molecular
Proliferating Cell Nuclear Antigen / metabolism*
Saccharomyces cerevisiae / enzymology

Substances

Proliferating Cell Nuclear Antigen
DNA
DNA Polymerase II

Grants and funding

R01 CA052040/CA/NCI NIH HHS/United States