Glucose stimulates insulin release without altering cyclic AMP production or inositolphospholipid turnover in freshly obtained human insulinoma cells

Biochem Biophys Res Commun. 1987 May 29;145(1):263-8. doi: 10.1016/0006-291x(87)91315-5.

Abstract

Glucose, forskolin, IBMX and carbachol all stimulated insulin release from freshly obtained human insulinoma cells. In these same cells, cellular cyclic AMP levels were raised by forskolin and IBMX but not by glucose and carbachol. On the other hand, of all the insulin secretagogues examined, only carbachol stimulated the formation of 3H-inositol trisphosphate in these cells. Thus, in these insulinoma cells, glucose apparently induces insulin secretion without altering cyclic AMP production or inositolphospholipid turnover.

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • Adenoma, Islet Cell / metabolism*
  • Carbachol / pharmacology
  • Colforsin / pharmacology
  • Cyclic AMP / metabolism*
  • Glucose / pharmacology*
  • Humans
  • In Vitro Techniques
  • Insulin / metabolism*
  • Insulin Secretion
  • Insulinoma / metabolism*
  • Kinetics
  • Pancreatic Neoplasms / metabolism*
  • Phosphatidylinositols / metabolism*

Substances

  • Insulin
  • Phosphatidylinositols
  • Colforsin
  • Carbachol
  • Cyclic AMP
  • Glucose
  • 1-Methyl-3-isobutylxanthine