Towards whole-body fluorescence imaging in humans

PLoS One. 2013 Dec 31;8(12):e83749. doi: 10.1371/journal.pone.0083749. eCollection 2013.

Abstract

Dynamic near-infrared fluorescence (DNIF) whole-body imaging of small animals has become a popular tool in experimental biomedical research. In humans, however, the field of view has been limited to body parts, such as rheumatoid hands, diabetic feet or sentinel lymph nodes. Here we present a new whole-body DNIF-system suitable for adult subjects. We explored whether this system (i) allows dynamic whole-body fluorescence imaging and (ii) can detect modulations in skin perfusion. The non-specific fluorescent probe indocyanine green (ICG) was injected intravenously into two subjects, and fluorescence images were obtained at 5 Hz. The in- and out-flow kinetics of ICG have been shown to correlate with tissue perfusion. To validate the system, skin perfusion was modulated by warming and cooling distinct areas on the chest and the abdomen. Movies of fluorescence images show a bolus passage first in the face, then in the chest, abdomen and finally in the periphery (~10, 15, 20 and 30 seconds, respectively). When skin perfusion is augmented by warming, bolus arrives about 5 seconds earlier than when the skin is cooled and perfusion decreased. Calculating bolus arrival times and spatial fitting of basis time courses extracted from different regions of interest allowed a mapping of local differences in subcutaneous skin perfusion. This experiment is the first to demonstrate the feasibility of whole-body dynamic fluorescence imaging in humans. Since the whole-body approach demonstrates sensitivity to circumscribed alterations in skinperfusion, it may be used to target autonomous changes in polyneuropathy and to screen for peripheral vascular diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Brain / physiology*
  • Fluorescence
  • Humans
  • Indocyanine Green*
  • Optical Imaging*
  • Skin / anatomy & histology
  • Skin / cytology
  • Skin / metabolism*
  • Whole Body Imaging / instrumentation
  • Whole Body Imaging / methods*

Substances

  • Indocyanine Green

Grants and funding

The technical equipment of this study was financed by the Sonnenfeld Stiftung (http://www.sonnenfeld-stiftung.de/) and NIRx Medizintechnik GmbH (http://www.nirx.net). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.