MspI polymorphic site within the factor IX gene. Localization of the site and an improved method for detection

Hum Genet. 1987 Jul;76(3):262-4. doi: 10.1007/BF00283620.


We have localized the position of the MspI polymorphic site that exists within the factor IX gene. The location of the MspI polymorphic site is within intron D, 1.9 kb upstream from the beginning of exon V and 4 kb downstream from a known polymorphic TaqI site. The use of a specific genomic probe simplifies the interpretation of the MspI polymorphism by reducing the number of non-overlaping DNA fragments to three bands; 2.4, 3.4, and 5.8 kb.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • DNA / genetics
  • DNA Restriction Enzymes / genetics*
  • Deoxyribonuclease HpaII
  • Exons
  • Factor IX / genetics*
  • Female
  • Genetic Linkage
  • Hemophilia B / genetics
  • Humans
  • Introns
  • Male
  • Nucleic Acid Hybridization
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length*
  • X Chromosome*


  • Factor IX
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease HpaII