Pharmacological studies on the antispasmodic, bronchodilator and anti-platelet activities of Abies webbiana

Phytother Res. 2014 Aug;28(8):1182-7. doi: 10.1002/ptr.5112. Epub 2014 Jan 6.


This study evaluated the antispasmodic, bronchodilator and anti-platelet activities of Abies webbiana to rationalize some of its folk uses in gut and airways disorders and inflammation. The crude extract of A. webbiana (Aw.Cr) caused a complete relaxation of both spontaneous and K(+) (80 mM)-induced contractions in isolated rabbit jejunum in a concentration-dependent manner. Aw.Cr shifted the Ca(++) concentration-response curves (CRCs) to the right, in a fashion similar to verapamil, confirming its Ca(++) channel blocking (CCB) effect. In isolated rabbit tracheal preparations, it caused relaxation of carbachol (1 μM) and K(+) (80 mM)-induced contractions comparable to verapamil suggesting that the bronchodilatory effect may possibly be mediated through CCB activity. Aw.Cr was found to be the inhibitor of both ADP- and epinephrine-induced aggregation of human platelets thereby suggesting therapeutic potential in this plant against thrombo-embolic conditions. The exhibited anti-platelet effect was observed at low doses against epinephrine as compared to ADP. This study confirmed the presence of spasmolytic activity in Abies webbiana through possible blockade of Ca(++) channels providing evidence for its folkloric use in gut and respiratory disorders in addition to anti-platelet activity.

Keywords: Abies webbiana; anti-platelet; calcium antagonist; gastrointestinal and respiratory disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abies / chemistry*
  • Adult
  • Animals
  • Blood Platelets / drug effects
  • Bronchodilator Agents / pharmacology*
  • Carbachol / pharmacology
  • Female
  • Humans
  • In Vitro Techniques
  • Jejunum / drug effects
  • Male
  • Parasympatholytics / pharmacology*
  • Plant Extracts / pharmacology*
  • Platelet Aggregation Inhibitors / pharmacology*
  • Rabbits
  • Trachea / drug effects
  • Verapamil / pharmacology
  • Young Adult


  • Bronchodilator Agents
  • Parasympatholytics
  • Plant Extracts
  • Platelet Aggregation Inhibitors
  • Carbachol
  • Verapamil