Attachment of Human Endothelial Cells to Polyester Vascular Grafts: Pre-Coating With Adhesive Protein Assemblies and Resistance to Short-Term Shear Stress

Physiol Res. 2014;63(2):167-77. Epub 2014 Jan 8.

Abstract

Cardiovascular prosthetic bypass grafts do not endothelialize spontaneously in humans, and so they pose a thrombotic risk. Seeding with cells improves their performance, particularly in small-caliber applications. Knitted tubular polyethylene-terephthalate (PET) vascular prostheses (6 mm) with commercial type I collagen (PET/Co) were modified in the lumen by the adsorption of laminin (LM), by coating with a fibrin network (Fb) or a combination of Fb and fibronectin (Fb/FN). Primary human saphenous vein endothelial cells were seeded (1.50 × 10(5)/cm2), cultured for 72 h and exposed to laminar shear stress 15 dyn/cm(2) for 40 and 120 min. The control static grafts were excluded from shearing. The cell adherence after 4 h on PET/Co, PET/Co +LM, PET/Co +Fb and PET/Co +Fb/FN was 22%, 30%, 19% and 27% of seeding, respectively. Compared to the static grafts, the cell density on PET/Co and PET/Co +LM dropped to 61% and 50%, respectively, after 120 min of flow. The cells on PET/Co +Fb and PET/Co +Fb/FN did not show any detachment during 2 h of shear stress. Pre-coating the clinically-used PET/Co vascular prosthesis with LM or Fb/FN adhesive protein assemblies promotes the adherence of endothelium. Cell retention under flow is improved particularly on fibrin-containing (Fb and Fb/FN) surfaces.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Vessel Prosthesis* / standards
  • Cattle
  • Collagen Type I / administration & dosage*
  • Endothelial Cells / physiology*
  • Humans
  • Polyesters* / standards
  • Saphenous Vein / cytology
  • Saphenous Vein / physiology
  • Shear Strength / physiology*
  • Stress, Mechanical*
  • Time Factors

Substances

  • Collagen Type I
  • Polyesters