Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: an update

Verena Stenico; Samanta Michelini; Monica Modesto; Loredana Baffoni; Paola Mattarelli; Bruno Biavati

doi:10.1016/j.anaerobe.2013.12.004

Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: an update

Anaerobe. 2014 Apr:26:36-40. doi: 10.1016/j.anaerobe.2013.12.004. Epub 2014 Jan 4.

Authors

Verena Stenico¹, Samanta Michelini², Monica Modesto¹, Loredana Baffoni¹, Paola Mattarelli¹, Bruno Biavati¹

Affiliations

¹ Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
² Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy. Electronic address: samanta.michelini2@unibo.it.

PMID: 24398432
DOI: 10.1016/j.anaerobe.2013.12.004

Abstract

A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.

Keywords: Bifidobacterium; Identification; PCR-RFLP; hsp60.

MeSH terms

Animals
Bacterial Proteins / genetics*
Bacteriological Techniques / methods*
Bifidobacterium / classification
Bifidobacterium / genetics*
Bifidobacterium / isolation & purification*
Chaperonin 60 / genetics*
Deoxyribonucleases, Type II Site-Specific
Humans
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length*

Substances

Bacterial Proteins
Chaperonin 60
Deoxyribonucleases, Type II Site-Specific
GGCC-specific type II deoxyribonucleases