Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart

Science. 1987 Jul 24;237(4813):411-5. doi: 10.1126/science.2440106.


Neuronal cells express a pp60c-src variant that displays an altered electrophoretic mobility and a different V8 peptide pattern relative to pp60c-src expressed in tissues of non-neuronal origin. To determine whether the neuronal form of pp60c-src is encoded by a brain-specific messenger RNA, a mouse brain complementary DNA (cDNA) library was screened with a chicken c-src probe and a 3.8-kilobase c-src cDNA clone was isolated. This clone encodes a 60-kilodalton protein that differs from chicken or human pp60c-src primarily in having six extra amino acids (Arg-Lys-Val-Asp-Val-Arg) within the NH2-terminal 16 kilodaltons of the molecule. S1 nuclease protection analysis confirmed that brain c-src RNA contains an 18-nucleotide insertion at the position of the extra six amino acids. This insertion occurs at a position that corresponds to a splice junction in the chicken and human c-src genes. The isolated c-src cDNA clone encodes a protein that displays an identical V8 peptide pattern to that observed in pp60c-src isolated from tissues of neuronal origin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology
  • Chickens
  • Cloning, Molecular
  • DNA / metabolism
  • DNA Restriction Enzymes
  • DNA Transposable Elements
  • Humans
  • Isoenzymes / genetics*
  • Mice
  • Neurons / enzymology*
  • Protein Kinases / genetics*
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins pp60(c-src)
  • Sequence Homology, Nucleic Acid
  • Species Specificity


  • DNA Transposable Elements
  • Isoenzymes
  • Proto-Oncogene Proteins
  • DNA
  • Protein Kinases
  • Proto-Oncogene Proteins pp60(c-src)
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M17031