Fast, simple and efficient supramolecular solvent-based microextraction of mecoprop and dichlorprop in soils prior to their enantioselective determination by liquid chromatography-tandem mass spectrometry

C Caballo; M D Sicilia; S Rubio

doi:10.1016/j.talanta.2013.10.043

Fast, simple and efficient supramolecular solvent-based microextraction of mecoprop and dichlorprop in soils prior to their enantioselective determination by liquid chromatography-tandem mass spectrometry

Talanta. 2014 Feb:119:46-52. doi: 10.1016/j.talanta.2013.10.043. Epub 2013 Oct 24.

Authors

C Caballo¹, M D Sicilia¹, S Rubio²

Affiliations

¹ Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, Agrifood Campus of International Excellence, University of Córdoba, Edificio Anexo Marie Curie, Campus de Rabanales, 14071 Córdoba, Spain.
² Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, Agrifood Campus of International Excellence, University of Córdoba, Edificio Anexo Marie Curie, Campus de Rabanales, 14071 Córdoba, Spain. Electronic address: qa1rubrs@uco.es.

PMID: 24401383
DOI: 10.1016/j.talanta.2013.10.043

Abstract

A simple, sensitive, rapid and economic method was developed for the quantification of enantiomers of chiral pesticides as mecoprop (MCPP) and dichlorprop (DCPP) in soil samples using supramolecular solvent-based microextraction (SUSME) combined with liquid chromatography coupled to mass spectrometry (LC-MS/MS). SUSME has been described for the extraction of chiral pesticides in water, but this is firstly applied to soil samples. MCPP and DCPP are herbicides widely used in agriculture that have two enantiomeric forms (R- and S-) differing in environmental fate and toxicity. Therefore, it is essential to have analytical methods for monitoring individual DCPP and MCPP enantiomers in environmental samples. MCPP and DCPP were extracted in a supramolecular solvent (SUPRAS) made up of dodecanoic acid aggregates, the extract was dried under a nitrogen stream, the two herbicides dissolved in acetate buffer and the aqueous extract directly injected in the LC-MS/MS system. The recoveries obtained were independent of soil composition and age of herbicide residues. The detection and quantitation limits of the developed method for the determination of R- and S-MCPP and R- and S-DCPP in soils were 0.03 and 0.1 ng g(-1), respectively, and the precision, expressed as relative standard deviation (n=6), for enantiomer concentrations of 5 and 100 ng g(-1) were in the ranges 4.1-6.1% and 2.9-4.1%. Recoveries for soil samples spiked with enantiomer concentrations within the interval 5-180 ng g(-1) and enantiomeric ratios (ERs) of 1, 3 and 9, ranged between 93 and 104% with standard deviations of the percent recovery varying between 0.3% and 6.0%. Because the SUPRAS can solubilize analytes through different type of interactions (dispersion, dipole-dipole and hydrogen bonds), it could be used to extract a great variety of pesticides (including both polar and non-polar) in soils.

Keywords: Chiral analysis; Dichlorprop; Liquid chromatography/tandem mass spectrometry; Mecoprop; Soil; Supramolecular solvent-based microextraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2,4-Dichlorophenoxyacetic Acid / analogs & derivatives*
2,4-Dichlorophenoxyacetic Acid / analysis
2,4-Dichlorophenoxyacetic Acid / isolation & purification
2-Methyl-4-chlorophenoxyacetic Acid / analogs & derivatives*
2-Methyl-4-chlorophenoxyacetic Acid / analysis
2-Methyl-4-chlorophenoxyacetic Acid / isolation & purification
Chromatography, Liquid / methods*
Limit of Detection
Pesticides / analysis
Pesticides / isolation & purification*
Reproducibility of Results
Soil Pollutants / analysis
Soil Pollutants / isolation & purification*
Solvents / chemistry*
Stereoisomerism
Tandem Mass Spectrometry / methods*

Substances

Pesticides
Soil Pollutants
Solvents
2,4-Dichlorophenoxyacetic Acid
mecoprop
2-Methyl-4-chlorophenoxyacetic Acid
dichlorprop