Single-step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction

Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.

Abstract

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

MeSH terms

  • Animals
  • Cell Line
  • Chloroform
  • Guanidine
  • Guanidines
  • Humans
  • Hydrogen-Ion Concentration
  • Mammary Glands, Animal / analysis
  • Phenol
  • Phenols
  • RNA / isolation & purification*
  • Rats
  • Solutions

Substances

  • Guanidines
  • Phenols
  • Solutions
  • Phenol
  • RNA
  • Chloroform
  • Guanidine